Coding

Part:BBa_K5242005

Designed by: Jianting Gong   Group: iGEM24_Tsinghua-M   (2024-09-24)


chk1

Chk1 has an alternatively spliced variant, Chk1-s, which skips exon 3 at the N-terminus. Chk1-s inhibits Chk1. In the normal cell cycle, Chk1-s interacts with Chk1, promoting the S-G2/M phase transition. When DNA damage occurs, phosphorylated Chk1 disrupts the Chk1-Chk1-s interaction, activating Chk1 to halt the cell cycle and promote DNA repair. Chk1-s and Chk1 expression levels are higher in fetal and cancer tissues than in normal tissues.

In our design, Chk1 is used as target RNA to be detected.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 164
    Illegal BamHI site found at 1090
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None